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mouse cdna library  (TaKaRa)


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    Structured Review

    TaKaRa mouse cdna library
    Mouse Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+kidney+cdna/Mouse+Kidney+Matchmaker+cDNA+Library+pACT2/pmc11668847-53-10-13
    Average 93 stars, based on 49 article reviews
    mouse cdna library - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Regulation of the renal-specific Na+–K+–2Cl− co-transporter NKCC2 by AMP-activated protein kinase (AMPK)
    Article Snippet: .. Mouse NKCC2N−term (amino acids 1–181) and NKCC2C−term (amino acids 813–1099) were PCR amplified from mouse kidney cDNA and cloned into the pEGFP-C2 vector (Clontech) to generate Nterminally tagged GFP–NKCC2N−term and GFP–NKCC2C−term and confirmed by sequencing. .. Substitutions of single amino acids were achieved using PCRbased mutagenesis reactions (QuikChange®, Stratagene) and were confirmed by DNA sequencing.

    Article Title: Regulation of the renal-specific Na + -K + -2Cl − co-transporter NKCC2 by AMP-activated protein kinase (AMPK)
    Article Snippet: .. The N-terminal (amino acids 1–181) and C-terminal domains (amino acids 813–1099) of NKCC2 were PCR amplified from total mouse kidney cDNA and cloned into the pEGFP vector system (Clonetech). ..

    Article Title: Regulation of the renal-specific Na + -K + -2Cl − co-transporter NKCC2 by AMP-activated protein kinase (AMPK)
    Article Snippet: .. Mouse NKCC2 N−term (amino acids 1–181) and NKCC2 C−term (amino acids 813–1099) were PCR amplified from mouse kidney cDNA and cloned into the pEGFP-C2 vector (Clontech) to generate N-terminally tagged GFP–NKCC2 N−term and GFP–NKCC2 C−term and confirmed by sequencing. .. Substitutions of single amino acids were achieved using PCR-based mutagenesis reactions (QuikChange®, Stratagene) and were confirmed by DNA sequencing.

    Article Title: Proteins having glucose transporter activity
    Article Snippet: .. Cloning of the cDNA Encoding the Mouse Kidney-derived Na+/Glucose Transporter Protein and Determination of the Base Sequence A PCR was carried out using mouse kidney cDNA (CLONTECH) as a template and 2 primers, i.e. primer 11 (SEQ ID NO: 17) and primer 12 (SEQ ID NO: 18). .. The reaction solution for the PCR contained 1 μl of the said cDNA as the template, 1 μl of Pfu Turbo DNA polymerase (STRATAGENE), 0.5 μM each of primer 11 (SEQ ID NO: 17) and primer 12 (SEQ ID NO: 18), 200 μM dNTPs, and 5 μl of the buffer solution attached to the enzyme product in a total volume of 50 μl.

    Amplification:

    Article Title: Regulation of the renal-specific Na+–K+–2Cl− co-transporter NKCC2 by AMP-activated protein kinase (AMPK)
    Article Snippet: .. Mouse NKCC2N−term (amino acids 1–181) and NKCC2C−term (amino acids 813–1099) were PCR amplified from mouse kidney cDNA and cloned into the pEGFP-C2 vector (Clontech) to generate Nterminally tagged GFP–NKCC2N−term and GFP–NKCC2C−term and confirmed by sequencing. .. Substitutions of single amino acids were achieved using PCRbased mutagenesis reactions (QuikChange®, Stratagene) and were confirmed by DNA sequencing.

    Article Title: Regulation of the renal-specific Na + -K + -2Cl − co-transporter NKCC2 by AMP-activated protein kinase (AMPK)
    Article Snippet: .. The N-terminal (amino acids 1–181) and C-terminal domains (amino acids 813–1099) of NKCC2 were PCR amplified from total mouse kidney cDNA and cloned into the pEGFP vector system (Clonetech). ..

    Article Title: Regulation of the renal-specific Na + -K + -2Cl − co-transporter NKCC2 by AMP-activated protein kinase (AMPK)
    Article Snippet: .. Mouse NKCC2 N−term (amino acids 1–181) and NKCC2 C−term (amino acids 813–1099) were PCR amplified from mouse kidney cDNA and cloned into the pEGFP-C2 vector (Clontech) to generate N-terminally tagged GFP–NKCC2 N−term and GFP–NKCC2 C−term and confirmed by sequencing. .. Substitutions of single amino acids were achieved using PCR-based mutagenesis reactions (QuikChange®, Stratagene) and were confirmed by DNA sequencing.

    Clone Assay:

    Article Title: Regulation of the renal-specific Na+–K+–2Cl− co-transporter NKCC2 by AMP-activated protein kinase (AMPK)
    Article Snippet: .. Mouse NKCC2N−term (amino acids 1–181) and NKCC2C−term (amino acids 813–1099) were PCR amplified from mouse kidney cDNA and cloned into the pEGFP-C2 vector (Clontech) to generate Nterminally tagged GFP–NKCC2N−term and GFP–NKCC2C−term and confirmed by sequencing. .. Substitutions of single amino acids were achieved using PCRbased mutagenesis reactions (QuikChange®, Stratagene) and were confirmed by DNA sequencing.

    Article Title: Regulation of the renal-specific Na + -K + -2Cl − co-transporter NKCC2 by AMP-activated protein kinase (AMPK)
    Article Snippet: .. The N-terminal (amino acids 1–181) and C-terminal domains (amino acids 813–1099) of NKCC2 were PCR amplified from total mouse kidney cDNA and cloned into the pEGFP vector system (Clonetech). ..

    Article Title: Regulation of the renal-specific Na + -K + -2Cl − co-transporter NKCC2 by AMP-activated protein kinase (AMPK)
    Article Snippet: .. Mouse NKCC2 N−term (amino acids 1–181) and NKCC2 C−term (amino acids 813–1099) were PCR amplified from mouse kidney cDNA and cloned into the pEGFP-C2 vector (Clontech) to generate N-terminally tagged GFP–NKCC2 N−term and GFP–NKCC2 C−term and confirmed by sequencing. .. Substitutions of single amino acids were achieved using PCR-based mutagenesis reactions (QuikChange®, Stratagene) and were confirmed by DNA sequencing.

    Plasmid Preparation:

    Article Title: Regulation of the renal-specific Na+–K+–2Cl− co-transporter NKCC2 by AMP-activated protein kinase (AMPK)
    Article Snippet: .. Mouse NKCC2N−term (amino acids 1–181) and NKCC2C−term (amino acids 813–1099) were PCR amplified from mouse kidney cDNA and cloned into the pEGFP-C2 vector (Clontech) to generate Nterminally tagged GFP–NKCC2N−term and GFP–NKCC2C−term and confirmed by sequencing. .. Substitutions of single amino acids were achieved using PCRbased mutagenesis reactions (QuikChange®, Stratagene) and were confirmed by DNA sequencing.

    Article Title: Regulation of the renal-specific Na + -K + -2Cl − co-transporter NKCC2 by AMP-activated protein kinase (AMPK)
    Article Snippet: .. The N-terminal (amino acids 1–181) and C-terminal domains (amino acids 813–1099) of NKCC2 were PCR amplified from total mouse kidney cDNA and cloned into the pEGFP vector system (Clonetech). ..

    Article Title: Regulation of the renal-specific Na + -K + -2Cl − co-transporter NKCC2 by AMP-activated protein kinase (AMPK)
    Article Snippet: .. Mouse NKCC2 N−term (amino acids 1–181) and NKCC2 C−term (amino acids 813–1099) were PCR amplified from mouse kidney cDNA and cloned into the pEGFP-C2 vector (Clontech) to generate N-terminally tagged GFP–NKCC2 N−term and GFP–NKCC2 C−term and confirmed by sequencing. .. Substitutions of single amino acids were achieved using PCR-based mutagenesis reactions (QuikChange®, Stratagene) and were confirmed by DNA sequencing.

    Sequencing:

    Article Title: Regulation of the renal-specific Na+–K+–2Cl− co-transporter NKCC2 by AMP-activated protein kinase (AMPK)
    Article Snippet: .. Mouse NKCC2N−term (amino acids 1–181) and NKCC2C−term (amino acids 813–1099) were PCR amplified from mouse kidney cDNA and cloned into the pEGFP-C2 vector (Clontech) to generate Nterminally tagged GFP–NKCC2N−term and GFP–NKCC2C−term and confirmed by sequencing. .. Substitutions of single amino acids were achieved using PCRbased mutagenesis reactions (QuikChange®, Stratagene) and were confirmed by DNA sequencing.

    Article Title: Regulation of the renal-specific Na + -K + -2Cl − co-transporter NKCC2 by AMP-activated protein kinase (AMPK)
    Article Snippet: .. Mouse NKCC2 N−term (amino acids 1–181) and NKCC2 C−term (amino acids 813–1099) were PCR amplified from mouse kidney cDNA and cloned into the pEGFP-C2 vector (Clontech) to generate N-terminally tagged GFP–NKCC2 N−term and GFP–NKCC2 C−term and confirmed by sequencing. .. Substitutions of single amino acids were achieved using PCR-based mutagenesis reactions (QuikChange®, Stratagene) and were confirmed by DNA sequencing.

    Article Title: Proteins having glucose transporter activity
    Article Snippet: .. Cloning of the cDNA Encoding the Mouse Kidney-derived Na+/Glucose Transporter Protein and Determination of the Base Sequence A PCR was carried out using mouse kidney cDNA (CLONTECH) as a template and 2 primers, i.e. primer 11 (SEQ ID NO: 17) and primer 12 (SEQ ID NO: 18). .. The reaction solution for the PCR contained 1 μl of the said cDNA as the template, 1 μl of Pfu Turbo DNA polymerase (STRATAGENE), 0.5 μM each of primer 11 (SEQ ID NO: 17) and primer 12 (SEQ ID NO: 18), 200 μM dNTPs, and 5 μl of the buffer solution attached to the enzyme product in a total volume of 50 μl.

    other:

    Article Title: Yeast strain for the production of proteins with terminal alpha-1,3-linked galactose
    Article Snippet: The resulting product was cloned into the pCR2.1 Topo vector (Invitrogen), sequenced, and named pRCD680.

    Cloning:

    Article Title: Proteins having glucose transporter activity
    Article Snippet: .. Cloning of the cDNA Encoding the Mouse Kidney-derived Na+/Glucose Transporter Protein and Determination of the Base Sequence A PCR was carried out using mouse kidney cDNA (CLONTECH) as a template and 2 primers, i.e. primer 11 (SEQ ID NO: 17) and primer 12 (SEQ ID NO: 18). .. The reaction solution for the PCR contained 1 μl of the said cDNA as the template, 1 μl of Pfu Turbo DNA polymerase (STRATAGENE), 0.5 μM each of primer 11 (SEQ ID NO: 17) and primer 12 (SEQ ID NO: 18), 200 μM dNTPs, and 5 μl of the buffer solution attached to the enzyme product in a total volume of 50 μl.



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